Type: HPLC Application #: 107210
Detection: UV, 260 nm, 0.08 AUFS Conditions: eluent A: 5 mM Tris/HCl pH 8.2, eluent B: 5 mM Tris/HCl pH 8.2 containing 0.5 M KCl, gradient: 5% B for 3 min, then in 7 min to 70% B flow rate 1 ml/min temperature ambient Authors: Macherey-Nagel Source: LC department
Details
Legend: Sample: standard mixture of nucleotide monophosphates (0.1 mM each), 1.
thymidine 5' monophosphate; 2. cytidine 5' monophosphate, 3. adenosine 5'
monophosphate, 4. guanosine 5' monophosphate, inject